I have a year old hatchery Buff Orpington who was always a bit bossy and flighty, nothing crazy but she was always the most "cautious" of my hens. Anywho lately ( the last 2 weeks) she's been puffing up and hissing at me when I catch her on the nest box, but she doesn't stay on it so I collect the eggs as usual and I don't think she's broody (is she pre-broody possibly). Today she puffed up at me in the run and hissed and feint charged me in the run, and generally acted super bossy. Here is a pic of her all puffed up.
She won't take her eyes off of me
2 chickens have recently been removed from the coop, could she just be acting out because her rooster is gone?