Natural flea and tick shampoo and vasoline didn't do much. I know I was supposed to apply another coat of vasoline a few days later. We're in a blizzard and cant really tend to the chickens how I wanted to. I can see their legs are red/pick and slightly swollen at the base of the feet. I know it's mites, but haven't seen any so far in the coop. Seems like the more time they spend in the coop the worse their legs look. Some are worse than others. It started with my favorite chicken and within a few weeks they all have the red/pink streak and pink between their toes. uggghhh. Please tell me how to use this neem oil.
Lots of people have different techniques for using it on this thread, so you'll have to decide what will work best for you. Also, I think there are different kinds of mites. I don't know if you have the scaly leg kind and if neem oil works for those. Maybe others can address that. Here is what I did, based on other advice here:
-Removed and threw out all bedding on coop floor and in nesting boxes, and sprinkled DE on bare surfaces, to go under bedding.
-Made a 50-50 mix of pure neem oil (not that garden spray stuff) and canola oil, put in a small spray bottle, and sprayed inside the coop, concentrating on crevices, cracks, and points where wood joins other wood. I also used paper towels to rub it all over the roosting bars (including underneath them). Try to do this on a sunny day when you can leave the coop open for several hours after you treat, as the smell is very strong and your chickens might be sensitive.
-Added new bedding.
-Made a much more diluted solution (a couple of drops of neem oil to a few tablespoons of canola oil) and put one small drop of this under each wing during the night while the birds were sleeping. I only did this once. (I had a friend come over and help me with that; I recommend a second person there to hold a flashlight while you handle the bird).
-Repeated the coop treatment above after one week.
Like you, I hardly saw any actual mites, but one had a vent that was slightly irritated which is why I thought there might be an excess mite load. After the first treatment, I saw a couple of dead ones on the roosting bar in the morning. Other people on the site wrote about looking into their coop during the night and seeing the mites; you may want to do that, as I think you generally don't seem them during the day unless you have a very severe infestation.
After all that, I have been using an occasional sprinkle of DE in the coop as a preventative (I don't think this will get rid of mites once they are there but might keep things under control when you don't have an actual problem), and I also frequently put fresh cut rosemary in the coop, which mites don't like but chickens seem to enjoy.
I also read that straw can harbor mites because it's hollow, so if you are using it you might want to switch to pine shavings. I had straw in the nest boxes but removed it all.
Hope you get rid of yours soon! I know it is very stressful.