Cowcreek, all four of my females were separated individually, and after about 2 or 3 minutes, they called out their two-syllable call. One held out for about 5 minutes, the little rascal - - it was a long five minutes. She sounded the male call for four of those minutes, too! Anyway, I'm sure that I have four females. I was just curious about what that soft, low-key one-syllable honk coming from my noisiest female might mean.
If you can separate yours out individually, you'll be able to tell pretty quickly who's male and who's female. When they're alone, they get stressed and call out. But that's only if you have the time to wait...the quiet guineas take longer to get worked up in my experience.
You probably have the males you want. The females would probably have made the 2 syllable call by now, looking for the male, but with the females able to make all male calls, it's hard to tell. But I think a female would make the female call trying to get the attention of males. I would :).
Peeps is the one who told me about separating them, and it works. She also told me that you can put a zip tie loosely around each one's leg to indicate male/female, but I have too few to do that since I can tell who's who. But unless you're building a flock for hatching specific colors, it doesn't really matter in my opinion. They're all good. :).