I'm not an expert but the 1st and 3rd chicks have more wattle development than the other chick, with the 1st chick specifically having really red wattles, which is a sign of having a young cockerel. However, this does not mean it's 100% a roo, some hens mature faster than others. I would just specifically watch and wait on the first and last chicks, and post pics in a couple weeks when they mature further. BTW I love the Frizzle Cochin! Good Luck!