If it's alive what have you done with the hole? Left it open or did you close it back up with something? And if you closed it, how did you do it?When incubating and that has happened to me I use a clean drill bit to make a hole in the air sack side of the egg that way I can look in and see if it's dead or alive. I have saved a few that way but usually they are already gone. But as a nurse we have a saying "you can't hurt something that's already dead". No movement candeling and water test I would try. If it's moving you haven't hurt anything if it's not you haven't hurt anything.
Okay, thank you so much! I'll make sure to try it.24 hours since the last chick hatched. I would do it if they were mine. They do still have the internal membrane. I always candle before I make the hole make a very small hole look for movement. If movement I return and check in an hour for progress. You can so see the internal membrane and if it's full of blood vessels. If no movement I will make the hole a bit bigger to confirm death.