Backstory to current situation. We had seven backyard hens. Last Thursday we woke to find that five had been attacked overnight--most likely a coon. I was concerned about only having two for company and warmth at night, so we did go same day to a farm and pick up three new young layers. So now, the two older and three smaller new girls. As expected, my sweet girls are rotten mean girls to the new pullets. So, during the day I have kept the new three in the winter run, and the older two in the yard, with access to the coop/nestboxes, but they can't peck or keep the new from eating. At night, I crate the older two just before dusk, allowing the new three to roost and putting the older two on the roosts after dark--otherwise they weren't allowing the new girls to roost. All has been going as fine as introducing new hens goes I guess until today. Earlier I went out to feed some sprouts and called my older pair as usual. Only one came. The other, was kind of puffed up, and not acting normally. She acted like she was going to poop, but nothing. When I picked her up and showed her the sprouts, she wasn't interested. Nor in water. Now, she's been on the roost in the coop for three hours. Is this something I should be concerned about?