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How long has it been since she was separated from dot? This time of year many chickens are also just starting up laying. If her comb is turning red and she is otherwise acting normal she most likely will start soon. Stress+Winter could very well cause her to stop laying.

Yes separated chickens stresses them out especially if they are alone. Is Loki now your only chicken? If so she most likely is very depressed. It's one thing if a chicken starts out a lone sometimes they manage, but going from being with another chicken to solitary confinement can really wreak...