I never culled or isolated any of my birds. Backyard chicken keepers usually prefer to repeat treatment and keep it under control rather than try to eradicate it which is difficult
You will have to treat the coop and chickens both to get rid of mites. The following link from the Mercks Vet Manual provides the correct treatment for each type of mite.What works for one mite doesn't work for all. Mites have built up a tolerance to permethrine in some areas making it...