How big is the coop?Do you see the roosters mating often?Same problem here. I checked at night with a flashlight, no mites or lice. Treated them anyway. It seems to only affect the dark colored birds. I have seven coops/runs. 32 hens and 4 roosters. The 2 coops that are affected are out in the open. The rest are inside a fence and they are fine. I have welded wire and hardware cloth around the bottom. Also concrete around the bottom to stop predators from digging. Sparrows and wrens can still get in. They carry mites and lice. Feathers are full of protein.Chickens will pick each other for that protein. I raised their protein and treated them for mites. It may be overcrowded . Might be the pecking order.The dark ones are bare around the vent but still laying. I need help too!