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I've dealt with E coli. I recommend you take a fresh fecal sample to a vet and have the vet send it off to a vet med school to be analyized. It shouldnt cost too much. You can also contact your agriculture department and they might recommend a place where you can send a fecal sample.
All poultry have E coli bacteria. It's when it gets out of control and spreads through their system is when it becomes deadly. There are many stressors that cause it to get out of control.
Symptoms include but are not limited to: Lethargic, not eating/drinking, listlessness, leading to the inability to stand or walk/roost, greenish/yellowish watery diarrhea. The green diarrhea is from not eating, most likely bile. The chickens health declines further til death. I had my 1 year old JG rooster put down after almost 2 months of various treatments; tube fed with powerful antibiotics and probiotics, all to no avail. E coli was officially verified to be the cause by the University of Georgia vet school.
I just recently brought one of my ladies to the local university vet school for what turned out to be an e. coli infection in her crop. She was given an antibiotic injection ever week for four weeks. They didn't say I needed to be concerned about the other hens at home. Yesterday was her final visit for a swab to be sure it is gone. Won't get the results for another week or so.