Yes I always leave for 24 hours at room temp to settle. I also set very upright in the incubator between the dividers and hatch in egg cartons. I like the egg carton method and have even started doing normal eggs this way. I feel it stops the hatched chicks bashing the eggs round the incubator. Plus with the eggs stationary in cartons I feel it's easier to spot and keep an eye on any pips and the progress of eggs.