They all look like pullets to me. Gladys' hackle feathers are kinda long, but nothing else indicates roo to me. They all look to have some EE in them (though I can't completely tell with Wilma and Nugget - do they have beards?), even Esther. Her feathers and shape scream EE to me, her head looks silkie-ish. Does she have any extra toes?
SUCH a cute flock, btw
SUCH a cute flock, btw