That is a really interesting question and I have NO idea!! I have just ordered hatching eggs from a hatchery in Florida (I am in SC) and the breeder contacted me today to say that the birds were "holding" their eggs...not laying because of the high winds and cold!
I went along with it because...
It is not unusual to have a very aggressive anti-social bird in a colony. They can be either sex, from my experience. It is best, as you did, to remove them altogether. As to introducing new birds to an established colony. That is a very tricky thing. Quail are very territorial and will not...
I have heard (lol) that the temperature in the incubator can influence the sex of birds. The lower the temp=more females. The higher the temp=more males.
Only ONCE in my many times of incubating and hatching Japanese quail has a lower (98.7F) temp yielded more females than males. Even though...