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The vent plug needs to come out at day 18, when you put them on lockdown. I've never hatched chickens but I have hatched quail. Quail eggs are rather dark with spots all over. It's pretty hard to candle them too. If I were you I would candle the eggs and measure the air sacs with a pencil...

Okay well you can do yourself a favor and trash those instructions. If you do a google search, pretty much every single website lists the same settings. 99.5-100.5, 45-55% humidity day 1-18, 65-75% humidity day 18-21. The vent plug does need to come out because the chicks need fresh air.

Congrats on your soon to be first hatch... What are you hatching..... chickens? Actually it appears your humidity may be too high (according to everything I have read). You want 40-50% humidity for the first 18 days, then you would raise the humidity to 65-75% for the final few days. Your...