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When you roll the egg around in your hand and the airsac stays up or continues to migrate to the upmost part of the egg it is detached, it should not move at all regardless if the egg is upside down. Quote:

It doesn't hurt to let them settle, I have set them both ways and seen no noticable difference. If the airsac is detached then they most likely are duds. I go 3 weeks down on the calender from the set date count backwards from 21 to 18 for lockdown and wait. Temp and humidity and stability of...

Actually an egg begins to develope after it is made or as some say laid. The incubation is slowed by the ambient air temperature but never really quits developing until it diesin about 10 days or so maybe a few more. To answer your question no there is no formal incubation done but they are...