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You only need to treat at the 10 day mark for the mites, then again 10 days later if you still have an infestation. It is so important to get rid of all the bedding each time you treat. I used to use lots of shavings and got cheap...I didn't want to throw away the shavings. Well they didn't...

Gjensen brings up a very important point- that you might require more than one retreatment. Definitely keep inspecting and retreat every 10 days until gone, no matter which treatment you choose. I have the liquid permethrin and it is good stuff. You have to peel the label to see the dilution...

Because of the life cycle of the mites, you need to retreat in 10 days. If you retreat earlier and don't do it at the 10 day mark, the eggs hatch and the reinfestation occurs. You also must get rid of all shavings in the coop and nest boxes for each treatment. I treat every 4 months year...