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If she is a food guarder put a second feeder out, and drive her nuts running from one to the other. They can do that with water also.

Many do it all the time. You should have enough room to make a pen in the coop so the new pullets can get acclimated and the ones you have now acquainted with them. A week or so should be enough time. If you have a large run as well of free range you can just let them mingle after the week.