For the record... they're pullets.
Female chickens are pullets until they're a year old. Then their hens.
male chickens are cockerels until they're a year old. Then they're roosters.
You're Isas are female colored, so; they can be nothing but female.
The wyandottes are very suspicious. its a little early for them... but their combs are large. Also, the gold laced is either very horribly laced, or a male, based upon the coloring.
I'd give it 2-3 more weeks on the...