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Nope, if they are truly broody, a mere temp drop will have no effect. Had a hen set and hatch in Jan/Feb....temps were in the single digit and teen F's.

Might try her again then.

So her second chance was just days from her first failure?

She's already had two chances, correct? I'd break her. You can still hatch her eggs, with an incubator or different broody hen.