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After checking around on some posts, I am pretty sure it means measuring their pubic area, holding the chicken upside down, and if the area looks a little swollen and you can place 3 fingers there, it might mean your pullet is the one that is laying. If your fingers can only stretch to 1 to 1...

Hi, my girls are 16 weeks old too, and I am new at all this, but what do you mean, "you measured their bums"?