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Yeah but given the vastly huger amount of air exchange in a coop than in a house, I can't see any reason to think it'd matter (for the coop, I mean) JMHO, Pat

MDF won't last in a coop, it will almost immediately begin to swell/distort/buckle/soften/disintegrate. Even if painted. Even if tiled over. Just don't, is all I can say. Use plywood, especially if you want to do a tiled floor (although that is not a low-maintenance option for a coop floor...)...