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Absolutely. A hen loses around 25% of her body weight during a normal broody period and if their broody state gets dragged out too long it can end up killing them. I've heard of this happening. Some hens are incredibly stubborn broodies and will simply stay broody for weeks and weeks, even if...

It sounds like two of your hens have gone broody. If you don't want them to hatch eggs for you you can break their broodiness by placing them in a wire bottom cage with only food and water for a few days.