My eyes aren't that good but I don't think so. Sometimes it's hard for me to see when they're right in front of me.
So the rooster was only with them one day - Thursday? Or 2 days, Thursday and Saturday?
That's not long enough. He may not have gotten around to all of them. Also, after a mating, semen is initially stored in the sperm storage ducts inside the cloaca. Then it has to make its way to the infundibulum where it enters more sperm storage ducts and fertilizes ovum that enter it. That usually takes a few days because an egg usually has to pass through the cloaca to squeeze out some of the semen so it makes that trek.
Usually, after mating, a hen has to lay an egg or two before ensuing eggs laid should be expected to be fertile.
That's because any egg laid, left the infundibulum 24 hours earlier and that location was the only opportunity to be fertilized.
