Nope! Once works for quite a while actually, hopefully someone here with more knowledge then me will tell us about how long... I don't know specifics but I do know it's not an every day thing. Is there a chance some of the infertile ones were gathered before the roo visited his hen?
Chickens are awesome!!
I had bought the eggs I was bating from a local breeder, so I don't know what was going on in the hen house. But I was soooo exited when the chicks began to peep through the eggs.
My kids were amazed.
They went back and forth to the bater all day watching the progress. I found it hard myself to keep waiting for them to come out by themselves. It took soooo long.
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How long did you leave the other eggs in after the four hatched. It's VERY possible that some or all of the others would have hatched within another three days, and maybe longer.