Yes! Here is the only way I could catch my roo (although last time we caught him, we ate him two days later - he had an attitude problem) or my white leghorn is to get them off the roost while they are sleeping.
I am telling you, they are SO docile. Husband picked the roo up off the roost using one hand underneath and captured roos legs between his fingers. The other hand goes on the back to hold the wings still. Flip them upside down really quick and check the vent. That is where the mites will be if you hav any. You will see them crawling there. You have to be quick because it is not good for them to be upside down for any length of time at all. If you do have mites, you will need some sort of pesticide dust, which you will dust directly onto their vents. Seriously, if you get them off the roost and be quick about it, it is like they don't even know what is happening.
BUT, I don't know if this is mites or not. Are they scratching any? Are you getting mysterious bug bites when you are around the chickens? I would probably separate him out for a while. Your hen might just be a bully.
That said, it isn't a bad idea to check for mites anyway.