Not necessarily. I recently set 11 eggs under a broody and on day 4 she got up off the nest for a few hours and wouldn’t sit back down so I had to move them under another broody. 9/11 eggs hatched, or 9/10 if you account for one of the eggs mysteriously disappearing. The 10th egg was infertile. Point is, there’s still a chance. I’ve hatched under worse circumstances, faulty incubators with bad temp/humidity, hens that got up for hours each day, letting the eggs go cold, ect. 
They might just be a bit behind in development. If I were you, I’d leave them in the bator until day 7-10 and candle them. If there’s veining, they’re alive, if they’re clear, they’re not.