New Way To Hatch And Grow An Chicken Embryo Try #10 ( Never Giving Up)

[pax12]

Going back to the idea when I first tried this project back in 09. The egg in the cup. On the botton I have clean egg shells for the calcium and the bag is the one that acts as a shell letting the Co2 out and the O2 in.

Have a cup that you clean very well, then put crushed egg shells at the bottom. Next crack the egg in and cover it with a kordon bag( The bag acts as a shell letting the O2 in and the Co2 out)
Then place it into the incubator and after 6 days I start tilting the cup so it acts like your turning it.
After 18 days poke a hole so it can breathe. Day 19 or 20 take off the cover and put something moist over the chick so it doesn't dry out ( pull up the chicks head so it doesn't cover it) then it should finish and hatch when its ready.



Watch the embryo grow live in front of your eyes.http://www.mycampage.com/stephenchickenandgreenhousecam

Other Tries At Growing An Embryo - http://www.youtube.com/playlist?list=PLFA6E52942972B4DD

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[pax12]

New Way To Hatch And Grow An Chicken Embryo Try #10 Day 0

 

[Pinky Macgyver]

Are you doing multiple so you know better if something went wrong with the process or if it was nature?

 

[pax12]

I have one going for now. Second one will be added in a few days.

 

[Pinky Macgyver]

Cool. I reckon it would be good to compare at the same stage though. Is there a reason isn't practical to do a few?

 

[pax12]

New Way To Hatch And Grow An Chicken Embryo Try #10 Day 1

Day 1:

6 to 10 h - First kidney-like cells (pronephros) begin to form.

8 h -Appearance of primitive streak.

10 h -Yolk sac (embryonic membrane) begins. Functions include: a) blood formation; b) yolk digestion; c) yolk absorption; d) food provision after hatching. Mesoderm appears; embryo oriented at 90° angle to egg's long axis; mesonephros begins.

18 h -Primitive gut begins; primordial germ cells appear in germinal crescent.

20 h -Vertebral column begins.

21 h -Appearance of neural groove, nervous system.

22 h -Appearance of first pair of somites (block-like segments) and head.

23 to 24 h -Blood islands, vitelline (yolk sac) circulation, blood, heart,blood vessels begin (2 to 4 somites).





Live Cam- http://www.mycampage.com/stephenchickenandgreenhousecam

Facebook Page - http://www.facebook.com/pages/Stephens-Chicken-and-Greenhouse-cam/149973155061889

 

[pax12]

New Way To Hatch And Grow An Chicken Embryo Try #10 Day 2

Live Cam- http://www.mycampage.com/stephenchickenandgreenhousecam
Facebook Page - http://www.facebook.com/pages/Stephens-Chicken-and-Greenhouse-cam/149973155061889

Day 2:

25 h -Appearance of eye; vertebral column visible; embryo begins to turn on left side (6 somites).

28 h -Ear begins (7 somites).

30 h -Amnion (embryonic membrane around embryo) begins. Primary function is to protect embryo against shock and sticking; also responsible for some albumen absorption. Chorion (embryonic membrane that fuses with allantois) begins; heartbeat begins (10 somites).

38 h -Cranial flexure and torsion evident; heartbeat moves blood (16 to 17 somites).

42 h -Thyroid begins.

48 h -Anterior pituitary and pineal glands begin to develop.

 

[Manningjw]

I was wondering if the acid in vinegar is able to diffuse through the membrane of the egg? If you could dissolve the egg shell while leaving the membrane intact without damaging the embryo inside I imagine it would develop. I know the water in vinegar is able to diffuse through the membrane now idea if the acid is as well, you would need to use something like albumin, saline or sugar to maintain a balance in osmotic pressure while dissolving the shell.

 

[pax12]

I tried that before and the vinegar got inside the membrane and it was no good.

 

[Manningjw]

Slightly dehydrate the egg, carefully remove shell where air sack develops, rehydrate slowly then try to hatch?

Really interesting what you are doing. How are you removing the shell and membrane without damaging the cell?