New Way to watch an Embryo Grow Try#5(Update Didnt Make It ) Eggtopsy

[pax12]

Im trying to find a new way to hatch and grow an embryo.

Project Started back in 2009.

Old videos of my experiments http://www.youtube.com/playlist?list=PL999FF131D6698E1E

I am going to try Kordon breathing bag... it should allow gas exchange but maintain moisture level. They are used for shipping fish and they have tested them with some fish for several weeks. The bag is a membrane that allows oxygen in, CO2 out, and keeps the liquid inside.

What im doing is a using a butter Containers to hold the yoke and white and using Kordon breathing bag to cover it, and putting the egg shell on the white so the the growing embryo can get calcium to grow.

The next is Im just using the bag to hold the yoke and white to see if it will grow.

Last Im just putting an egg in the bag to see if it can live up to term, and to see if the bag can receive oxygen to see if the embryo can live under the perfect condition of the egg, with out me doing anything to it. So this test is just to see if the bag can allows oxygen in, CO2 out and to see if the embryo can grow in the bag and inside its own shell. when it gets late into hatching I will poke holes in the bag so it can get more oxygen

Web Cam- http://www.mycampage.com/stephenchickenandgreenhousecam

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Trial Run

Day 1- http://www.youtube.com/watch?v=-mbbWXj76gU
Day 2- http://www.youtube.com/watch?v=rlpFi65MwAc
Day 3- http://www.youtube.com/watch?v=w-nm2OWClQM
Day 4- http://www.youtube.com/watch?v=J_-iGP5mWLY
Final Test - http://www.youtube.com/watch?v=d1s6qywYc5E

New Way to watch an Embryo Grow Due 1/8/12

Day 0- http://www.youtube.com/watch?v=wCYttJoCvlk
Day 1- http://www.youtube.com/watch?v=gLlEB25NF60
Day 2- http://www.youtube.com/watch?v=KXUPJM0vZpQ
Day 3- http://www.youtube.com/watch?v=S0YaP30KOJM
Day 4- http://www.youtube.com/watch?v=8paD9JmF3-Y
Day 5- http://www.youtube.com/watch?v=E-Z3oOgu7Rk
Day 6- http://www.youtube.com/watch?v=_IaCn8aqc4U
Day 7- http://www.youtube.com/watch?v=JI7nJGpjb0w
Day 8- http://www.youtube.com/watch?v=VOiznrzL1dk
Day 9- http://www.youtube.com/watch?v=QO6yIG8BKDM&feature=youtu.be
Day 10- http://www.youtube.com/watch?v=Oy9oqGuX-q4&feature=youtu.be
Day 11- http://www.youtube.com/watch?v=woghwYfXu74
Day 12- http://www.youtube.com/watch?v=5ikPdumqSpU
Day 13- http://www.youtube.com/watch?v=7GPnbcGeYkY
Day 14- http://www.youtube.com/watch?v=zsS5oZlCp7M&feature=youtu.be
Day 15- http://www.youtube.com/watch?v=Pzyr34qUe2Q&feature=youtu.be
Day 16- http://www.youtube.com/watch?v=yVhiT_o1tsU
Day 17- http://www.youtube.com/watch?v=RK8zkUELV6M
Day 18- http://www.mycampage.com/stephenchickenandgreenhousecam
Dat 19- http://www.mycampage.com/stephenchickenandgreenhousecam
Eggtopsy -

 

[pax12]

During Incubation:

Day 1:

6 to 10 h - First kidney-like cells (pronephros) begin to form.

8 h -Appearance of primitive streak.

10 h -Yolk sac (embryonic membrane) begins. Functions include: a) blood formation; b) yolk digestion; c) yolk absorption; d) food provision after hatching. Mesoderm appears; embryo oriented at 90° angle to egg's long axis; mesonephros begins.

18 h -Primitive gut begins; primordial germ cells appear in germinal crescent.

20 h -Vertebral column begins.

21 h -Appearance of neural groove, nervous system.

22 h -Appearance of first pair of somites (block-like segments) and head.

23 to 24 h -Blood islands, vitelline (yolk sac) circulation, blood, heart,blood vessels begin (2 to 4 somites).


Day 2:

25 h -Appearance of eye; vertebral column visible; embryo begins to turn on left side (6 somites).

28 h -Ear begins (7 somites).

30 h -Amnion (embryonic membrane around embryo) begins. Primary function is to protect embryo against shock and sticking; also responsible for some albumen absorption. Chorion (embryonic membrane that fuses with allantois) begins; heartbeat begins (10 somites).

38 h -Cranial flexure and torsion evident; heartbeat moves blood (16 to 17 somites).

42 h -Thyroid begins.

48 h -Anterior pituitary and pineal glands begin to develop.


Day 3:

50 h -Embryo turns on left side; allantois (embryonic membrane that fuses with chorion) begins. Functions of chorioallantois are: a) respiration; b) albumen absorption; c) absorption of calcium from shell; d) storage of kidney excretions.

60 h -Nasal pits, pharynx, lungs, anterior limb buds begin.

62 h -Posterior limb buds begin.

72 h -Middle and outer ear, trachea begin; amnion completes growth around embryo.


Day 4: Tongue and esophagus begin; embryo separates from yolk sac; allantois grows through amnion; contractions occur in amnion wall; adrenal development begins; pronephros (nonfunctional kidney) disappears; metanephros (definitive or final kidney) begins; proventriculus, gizzard, ceca, large intestine begin. Pigment visible in eye (dark eye).


Day 5: Reproductive system and differentiation of sex appear; thymus, bursa of Fabricius, duodenal loop begin; chorion and allantois begin to fuse; mesonephros begins to function; first cartilage present.


Day 6: Beak appears; voluntary movement begins; chorioallantois (chorion fused with allantois) lies against shell near large end of egg.


Day 7: Digits appear; comb growth begins; egg tooth begins; melanin produced; absorption of mineral from shell begins. Chorioallantois is attached to inner shell membrane and growth around the inner surface is progressing.


Day 8: Father tracts appear; parathyroid begins; bone calcification begins.


Day 9: Growth of chorioallantois about 80% complete (still open at small end); mouth opening appears.


Day 10: Beak begins to harden; digits completely separated.


Day 11: Abdominal walls established; loops of intestine begin to protrude into the yolk sac; down feathers visible; comb and wattles visible; claws and scales appear on toes; mesonephros reaches maximum level of function, then begins to degenerate; metanephros begins to function.


Day 12: Chorioallantois completes enclosure of egg contents; embryo water content begins to decrease.


Day 13: Cartilaginous skeleton is relatively complete; embryo heat production and oxygen consumption begin to increase rapidly.


Day 14: Embryo begins to turn head toward large end of egg; long bone ossification becomes rapid. Turning of egg no longer essential.


Day 15: Intestinal loops easily seen in yolk sac; contraction of amnion ceases.


Day 16: Beak, claws, and scales relatively cornified; albumen is practically gone and yolk increasingly important as food source; down feathers cover body; intestinal loops begin to retract into body.


Day 17: Amniotic fluid decreases; embryo positioning head toward large end, toward right wing with beak toward air cell; definitive feathers begin.


Day 18: Blood volume decreases, total blood hemoglobin decreases. Embryo should be in proper position to hatch: embryo's long axis the same as long axis of egg; head in large end of egg; head to right and under right wing; beak pointed toward air cell; feet toward head.


Day 19: Intestinal loop retraction complete; yolk sac begins to enter body cavity; amniotic fluid (swallowed by embryo) disappears; beak may pierce air cell and lungs begin to function (pulmonary respiration).


Day 20: Yolk sac completely drawn into body; air cell pierced, followed by functioning of pulmonary respiration; embryo makes sounds; chorioallantoic circulation, respiration, and absorption decrease; embryo may pip shell.


Day 21: Hatching process: chorioallantoic circulation ceases; embryo breaks shell over air cell with egg tooth; embryo slowly rotates in egg counterclockwise, chipping and breaking shell as it does; embryo kicks and attempts to straighten neck, pushes shell open; kicks free of shell, rests, straightens, dries.


>Day 21: Some embryos are unable to hatch but survive beyond the normal hatching time.

 

[Sphinx]

I've seen you make several attempts at this.

I'm curious- how far have you gotten an egg to develop thus far? What's your record?

 

[pax12]

Keep it alive for a week before it got bumped and the yoke broke.

 

[Melissa Rose]

Woowwwww you and your experiment are truly impressive!!! Your parents must be so very proud of you ! Happy Holidays to you and your family!!!!

 

[just2rosey]

This is interesting and I think you have a good shot at making it work, but I think you may need another modification. It appears that the egg white is a very thin layer in the bottom of your container. There is not sufficient depth for the embryo to remain fully covered by the egg white, which will be necessary for the egg to fully develop. I think you need a container with a smaller diameter that will more closely mimic the shape of the egg, a small measuring cup perhaps? Just a thought. Good luck I can't wait to see how it goes.

 

[pax12]

Quote:
They are haha, thanks and Happy Holidays to you .

 

[pax12]

Ok thanks.


Day 2 video coming soon

 

[pax12]

I also have fertile eggs coming this week!

So far everything is going great with the testing.

Day 2-

 

[pax12]

everything is going great.

Day 3-