DIRECTIONS FOR MD-VAC® CFL
1. Vaccinate healthy one-day-old chicks only.
2. Avoid early exposure of chicks to Marek’s virus challenge.
3. Use Zoetis Inc. diluent only. Store diluent at not over 27°C (80°F).
4. 1,000 Doses
Rehydrate only 1 vial (1,000 doses) with 200 mL of diluent.
2,000 Doses
Rehydrate only 1 vial (2,000 doses) with 400 mL of diluent.
5. Remove the central tab of the aluminum seal of the vaccine vial, leaving the outer ring intact. Sanitize the rubber stopper with alcohol. Also, sanitize with alcohol the rubber stopper of the bottle of diluent.
6. Using a sterile syringe and needle, withdraw 3 mL diluent. Then, insert needle through stopper of vaccine vial and discharge syringe contents.
7. Remove syringe and needle. Mix gently until vaccine is dissolved. Pull back on syringe plunger to admit 2 or 3 mL of air. This is very important.
8. Reinsert needle into vaccine vial. Barely penetrate the stopper. Expel the air into the vial (to break vacuum).
9. With syringe and needle still in place, invert the vial and pull back plunger so that all the contents (the rehydrated vaccine) are drawn from vial into syringe.
10. Now, insert the needle into diluent and expel syringe contents into the diluent. Gently swirl until the mixture is uniform. The vaccine is ready for use.
11. During vaccination, maintain the rehydrated vaccine (in the diluent bottle) at 70° to 80°F (21° to 27°C). If the temperature cannot be held as low as 80°F (27°C), place the diluent bottle containing the rehydrated vaccine in an ice bath.
12. For vaccination, an automatic syringe with 22- to 20-gauge needles, 3/8-inch to 1/2-inch in length, is recommended. Make certain that all equipment is sterilized and change needles frequently.
13. Inject each chick subcutaneously with 0.2 mL of the vaccine.
14. Use all the vaccine from 1 vial within 1 hour after rehydrating. Do not save any vaccine that has been rehydrated. Burn vaccine containers and all unused contents.