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We use Vircon S. Mix per instructions and then spray every part of the coop. We even sprays some of the ground around the coop as well. Keep all animals away while it is still wet. After it dried we allowed the coop to air out for a number of days. Haven't lost a bird since then and that was 2...

I hate to say it but when we had Marek's we lost our entire flock. We left the coop empty for about 6 months and then completely scrubbed it with a Bacteroide twice. That was 2 years ago and we have had no problems since. All our chickens since have been vaccinated.