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No,one has it will spread to the others.Do they have dusting spots?Would use permituian spray as well.Treat twice every time you clean coop.I clean my coop every Sunday and treat my cooo once a year.Treated my coop with pemrethin spray last Sunday and today.Will not treat again unless noticed...

Try going in at night with a flash light and seeing if you can see any crawling on roosts and etc