As
@JustBabyMargo said, these are your only pullets.
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I try not to be judgmental without knowing the full story but I'm going to be a bit harsh. You absolutely need to rehome at least three (and four would probably be smarter) of your cockerels. It is at the very least neglectful to all of your flock and perhaps even abusive to allow this ratio of birds. Roosters should ideally have about 10(!) hens a piece. You either need about 38 more hens ASAP or rehome all your boys. It isn't fair to their quality of life. Your hen is hiding on the roost because she would get injured and could even get killed because you have such a large number of roosters with only two hens. I advise you as soon as possible to separate out your cockerels and pullets. That will minimize conflict between the cockerels so they aren't fighting over girls and give your poor pullets a better quality of life. To be completely honest, my heart breaks for your girls.

It will make your chicken keeping experience less stressful in my opinion to keep a female-only flock. To keep a rooster you have to have planning and vigilance and if you're unprepared, you wind up with situations like your own.

I'm sorry for being stern but the with the age of these birds and your problems you've explained, this is not just a question of what gender they are and move on. This is down to the wire, separate and rehome now.