In my experience - which is limited with multiple breeding pens- the hens don’t appreciate their Roo being taken away. It becomes sort of a family group and when you break that up there could be consequences. Hence I leave my groups together if at all possible. The thing that allows that is that my Roos are chosen to be specifically good to the hens. Some don’t care for me but as long as they are good with the flock, they stay together. I have one Cream Legbar that may have been traumatized when I removed her Roo from my breeding plans. She was given time off and placed with the son of the Roo she’d been with- along with another hen from the same pen- and she has completely changed personality and lost weight. She is very shy now. The second Roo is no longer part of my breeding plans either. I’ve entirely shifted away from Cream Legbars. This hen is the only hen I have from one specific line. So to recap… I don’t remove roosters unless absolutely necessary. I also have very different ideas of my chickens and projects and understand my reply may be a minority opinion. Chickens do bond to each other.