Just to be clear I'll go about it this way. It takes about 25 hours for an egg to go through the hen's internal egg making factory. That egg can only be fertilized in the first few minutes of that journey. That means if a successful mating takes place on Monday, Monday's egg will not be fertile. Tuesday's egg might be but don't count on it. Wednesday's egg will be fertile. Notice that this is after a successful mating. A rooster does not necessarily mate with every hen in the flock every day, but he does not have to.
In the last part of the mating act the rooster hops off. His part is done. The hen then stands up, fluffs up, and shakes. This gets the sperm in a container near the point the egg starts it's internal journey. That sperm can stay viable in that container from 9 days to over three weeks. Most of us use two weeks as the length of time it stays viable and get pretty good results. A lot of people use a three week window for how long they need to clean out a hen before they are assured the new rooster will be the father. In isolated cases the sperm has lasted over three weeks so to be 100% sure four weeks is better.
it sounds like you are not dealing with hens and roosters but instead have pullets and cockerels. There are some considerations there. I sometimes incubate a pullet's first eggs. They can hatch. But I find my hatch rate is a little lower and the chicks have a higher mortality rate than if the eggs are from pullets or hens that have been laying a while. An egg has to be put together about perfectly to hatch a healthy chick. When pullets first start to lay they can take a while to get all the kinks out of their internal egg making factory. Those kinks can be more than just the weird things that are noticeable. The first eggs are small also compared to what they will be a month later. The chicks that do hatch out of them are pretty small and maybe a bit weaker. I still set pullet eggs and sometimes get some good results but I find waiting until the pullet has been laying for a month at least before I incubate eggs gives me a much better hatch.
Same type of thing with cockerels. The young ones are not perfect but they can be energetic. They only have to hit the target once every two weeks anyway. If your pullets are already laying and your cockerels are already trying to mate, I'd think if you wait three to four weeks for the sperm to clear up you will be good to go. You might possibly get marginally better results in hatch rate and survivability if you wait a bit longer but the difference is not worth the wait to me. From what I've seen it is insignificant.