It's possible, although probably not recommended, unless you are using an incubator that is designed to do it. Your best bet is to run 2 incubators, one for incubating and one for hatching. Have the second incubator up and running by day 18 of the first set, and transfer them to it so you can lock them down keep the humidity up. Meanwhile, it's business as usual for the second set for another week.
That said, I have pulled it off with one incubator in a pinch. I stuck a couple of eggs in the incubator to test fertility, and was 99% sure they'd be clear, but much to my surprise three started developing. They were 10 days behind the other eggs, and I didn't hold out much hope for them, but I couldn't bring myself to toss them and I just let them be. Since there were only 3, it only took a second to turn them during the lockdown, and my hygrometer/thermometer barely changed. This might not be the case if they have a dozen eggs to turn, though. I was worried about the high humidity, and bacteria introduced during the hatch, but 2 of the 3 hatched and were fine. The 3rd quite around day 15. It did not seem to affect my first hatch, which resulted in 16 out of 17. As soon as the hatch was over, I removed the eggs, set the top aside still plugged in, as quickly as possible scrubbed the bottom half, put it back together and got the eggs back in it. Hens get off their nest during incubation to eat & drink for as much as 20-30 minutes, so if you can do this FAST and get the temp/humidity back up you may pull it off.
I am in no way encouraging anyone to try this - I believe my chicks hatched against the odds - but I hope this information helps you get through the hatch that is already in your incubator.