With our nice warm evenings here in Texas, next time do it after they go to roost, easy breezy. There should be no need to repeat treatmentI just used the same bottle what was left from spraying chicken and sprayed coop. It was the 16oz dilut. I only used about half maybe more on chickens and I think I got them pretty good. What was left in spray bottle I used in coop. All my chickens have feathered legs and the rooster legs still look a little pink so I am going to wait the 14 days and do it all again. It took me from 730 am to 330 pm. Was a chicken rodeo trying to catch hens that don't want to be picked up.