Testosterone certainly lends a brighter red if that's what your asking. IE: pink hen combs versus red rooster combs.I know this is old, but is there any possibility the blue faded because it was hormonal? What I mean is, my Buff Orpington rooster had a completely dark purple comb when his hormones started kicking in. As he got older it all went back to red. He would get some purple from time to time, but definitely not full comb or as dark as when he was young. Guessing that has something to do with the melanin being more spread out as they get bigger as you said as well.