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Ok, so right now I have two roosters in one pen with all of the hens/pullets due to not enough room right now. They are not separated at all so they are both breeding same hens and what not. When I separate them and keep them separated with Rooster A in one pen and rooster B in another and they start breeding all the hens in each of their pens, will the other roosters semen fade out? If so, will it take up to a month?
Using Jerrys advice, I'd think you could wait just two weeks.