This is not a yes or no answer, but a maybe. It can possibly affect them. If the air cell migrates it can really mess up a chick when it hatches. If you incubate them wrong side up, it will definitely make a huge difference in hatch rate. The chick just can’t get oriented with the air cell and can’t do an internal pip. The chick will probably fully develop but die in the shell.
But you have not been incubating them and the chicks have not started to develop. What I’d expect to happen with yours is that you will not have a total disaster because of this, but you will probably get a lower hatch rate, especially with the ones that have been stored this way the longest. One or two days, it probably doesn’t matter. Six or seven days, it really could. It’s not that at a certain time period they all go bad. The longer they are stored that way the less your odds of success become.
You can try to incubate them and see what happens. You could wind up with a pretty good hatch. But if you can, I think I’d eat the older eggs and keep saving for a few more days. I think you’ll be happier at the end of the day.
Good luck!