That's the problem with staggered hatches. The eggs you put in 3 days later actually would not require turning when the first ones go into lockdown. An egg does NOT need to be turned after 2 weeks because the chicken is formed and can independently move. I actually stop turning my eggs at the end of day 13 so that days 14/15 when they make the turn to the air cell end, they are not being moved.
Now the last one, is your problem, because that is still going to need to be turned and it's going to need less humidity, but in this case you would do what is best for the majority, which is going to be the first ones. Yes, you need to take the turner out when you go into lockdown for the first 9. It wouldn't hurt if you have your humidity up to continue to turn the last egg. I keep my humidity at 75% during lockdown and hatch because I often open the incubator during hatch, so my philiosophy would be, if you have a bator that holds good temps and recovers humidity well and you are comfortable doing so, go ahead and continue turning. After the first 9 hatch the next ones will be in lockdown, so to stop altogether is more likely to affect the egg than to continue turning through the hatching. (Again, providing that you keep the humidity up in the bator during hatch time.
If you are not comfortable opening during hatch or your bator has problems recovering humidity, then your only other option is turn until the first pip and then stop and see what happens.
How many days are there between the second batch and the last one?