Coturnix quail? How old are they? Pretty sure you'll never get fertile eggs you moved the roos to a different cage..... not sure why you separated. If coturnix quail you should keep them in 1:4 male to female ratio + or - 1 female. 2 roos and 3 hens will not work out well for any of the quail, females will get overbred once the males start breeding and the males will likely fight.