how do I preserve an embryo for classroom use?

Discussion in 'Incubating & Hatching Eggs' started by OCchickens, Feb 16, 2009.

  1. OCchickens

    OCchickens Chillin' With My Peeps

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    Jul 19, 2008
    Brea, California
    Hi -- just wondered whether anyone had a suggestion of how to preserve a chick embryo for classroom use. These are from our recent hatch eggtopsies -- no chicks were killed for this purpose!!
     
  2. silkiechicken

    silkiechicken Staff PhD Premium Member

    From what I would do.... which may not necessarily be the best or right way...

    Depends on how big it is. If it's really small, like dime size, you can preserve it in 4% PFA by just dropping it in like 20 ml. One or two changes of PFA would be good to ensure you have enough in there to preserve it. If it's bigger, you might have to do more changes, maybe every 24 hours, for about a week to make sure all the tissues are penetrated. Then you just leave it floating in the liquid for how ever long you want in a no food allowed fridge... however... PFA isn't something you want in your house hold fridge lest you increase your chances of cancer.

    You can try using 100% etOH to preserve it, but it will distort the embryo a bit more because it dehydrates the tissues rather than crosslinks them. Similar to the PFA, soak and change liquid for a day to a week or so and leave suspended. 100% ETOH won't hurt anyone and can be kept at room temperature. However, before the tissues are fixed, I suggest doing all the wash steps in a refrigerator.

    Good luck!
     
  3. monarc23

    monarc23 Coturnix Obsessed

    8,672
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    Jul 18, 2008
    Indiana, Pennsylvania
    frameldahide? (sp?) [​IMG]
     
  4. daisychick

    daisychick Incubator Tetris Master Consultant

    Feb 8, 2009
    Colorado
  5. rebelcowboysnb

    rebelcowboysnb Confederate Money Farm

    Vodka or tequila work. Ive seen bourbon used.
     
  6. Teach97

    Teach97 Bantam Addict

    Nov 12, 2008
    Hooker, OK
    Formalin works - basically diluted formaldahyde and they don't freak as much...still not touch friendly and such...you could use that to fix it and then put in alcohol...
     
  7. OCchickens

    OCchickens Chillin' With My Peeps

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    Jul 19, 2008
    Brea, California
    Wow, I'm impressed with how much people know about this. What is PFA? Sounds scary in terms of the cancer stuff. What about formalin -- is is the same thing?

    I'll check out the Illinois link.
     

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