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Only if you want to keep setting eggs all spring and all summer, otherwise, just 4 weeks plus however long you're collecting eggs for hatching.
I sometimes separate roos for one reason or another, usually they're on death row waiting to go to freezer camp, and I separate them because they're harassing the hens to death.
I don't have any way to keep them from seeing the hens through the wire, but after the first few days they usually stop fighting. they figure out they ain't gonna get the girls anyway. Right now I have 3 young dark Cornish cockerels in a pen alone, because I just got them, and I have too many roos running loose as it is. And new babies all over, adding more males right now, might get some of the chicks killed in the cross-fire. So, they will remain penned until some of the other males are gone. Anyway, they were fighting the first 2 days, but they don't seem to be doing that now.