No, once you've taken the contents out of the egg you can't put it back in and incubate it. If you want to hatch some eggs take a few sample eggs from your flock and check them for fertility, if most of them or all of them are fertile chances are the rest of the eggs laid around the same time will be too. A hen will lay fertile eggs for roughly 3 weeks after one successful mating, so if you have a rooster with the hens and he's doing his job you should have plenty fertile eggs to hatch. If you candle the egg around 7 days into incubation you should be able to see if it's developing. And that is the only way of checking fertility without breaking the egg open.