Inseminating Infertile Eggs- PLEASE Help!

Discussion in 'General breed discussions & FAQ' started by Redcatcher, Jun 28, 2011.

  1. Redcatcher

    Redcatcher Chillin' With My Peeps

    May 7, 2010
    At My Desk!
    I know this is a long shot...But since I have nothing to lose, I thought I would ask anyway. I knew someone years ago whose job involved fertilizing infertile turkey eggs. Apparently this was an alternative to traditional AI. From what I can recall, he said this was done with a syringe through the shell of the egg. I have three eggs that my gynandromorph chicken laid before she died. I realize that the offspring would very likely be normal but does anyone know what would be involved in fertilizing them?
     
  2. geosheets

    geosheets Chillin' With My Peeps

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    Jun 8, 2009
    Ohio
    Found this....

    The opening can be made anywhere in the shell that effects viable fertilization, but is typically made in an area of the shell that is near the germinal disc. While an egg may be manipulated so as to place the germinal disc at different regions of the egg, the germinal disc in a newly oviposited egg is located at the large end of the shell, which overlies the air cell adjacent to the yolk. Once an opening has been created in the shell, the sperm sample is preferably delivered by introducing the needle, pipette, etc., through the air cell and beneath a membrane lying below the air cell (inner shell membrane). The sperm number can be increased or decreased, depending on where and in what form the sperm are administered. In a further preferred embodiment, the sperm sample is delivered into the egg using a needle. In nature, the sperm cells must penetrate the inner perivitelline membrane and fuse with the oolema for successful fertilization to occur. With IOF, the sperm cells must also penetrate the outer perivitelline membrane before successful fertilization can occur. To increase the fertilization efficiency, one can treat the OPL. Any treatment which rendered the OPL or yolk membrane more permeable to sperm could be utilized, for example, a non-toxic acid, a proteolytic enzyme or physical abrasion.

    In one embodiment, the needle, pipette, etc., is advanced through the shell at an angle of approximately 15°, penetrating the membrane lining the shell. In a method of the invention, the needle, pipette, etc., can be advanced through the air cell, until it meets the inner shell membrane. A person practicing the method of the invention will know that the tip of the needle, pipette, etc., has encountered the membrane when slight resistance to further advancement of the tip is felt. As the tip is gently advanced, the resistance from the membrane gives way and the tip is allowed to barely penetrate the membrane. The sperm sample can then be delivered into the egg, adjacent to a region of the membrane and that is adjacent to the germinal disc. Therefore, the sperm can be delivered just under the membrane, a process known as intracytoplasmic sperm injection (ICSI). Typical volumes of the sperm sample are as small as 0.005 ml or as large as 0.10 ml. A typical volume of injected sperm sample is about 0.01 ml.

    Preferably, to prevent contamination of the egg and death of an embryo, the opening in the shell is sealed. A non-toxic adhesive can be applied directly to the opening in the shell to seal it. Alternatively, a piece of eggshell can be used as a patch to close the opening and may be attached to the shell with a non-toxic adhesive. In one embodiment, the non-toxic adhesive is Elmer's[​IMG] glue. In another embodiment, the adhesive is silicone sealant. Moreover, any “tissue glue” can also be used to seal the shell. A “tissue glue” is a sterile, non-toxic adhesive used during surgical, operative procedures to bind tissues together.
     
  3. BeardedLadyFarm

    BeardedLadyFarm Chillin' With My Peeps

    May 31, 2009
    Cobleskill NY
    WOW! I had no idea this was possible. It's giving me ideas...
     
  4. Orange Ribbon

    Orange Ribbon Chillin' With My Peeps

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    121
    Apr 14, 2011
    Quote:Me too! Think of the possibilites! Think of the POWER OVER LIFE! THINK OF THE FAME!!! HAAHEEHAHAHHAH!!! [​IMG]

    Sorry, I got excited.
     
  5. Redcatcher

    Redcatcher Chillin' With My Peeps

    May 7, 2010
    At My Desk!
    Quote:Thank you geosheets for this information! This is totally fascinating. I searched the internet to no avail and was beginning to think that my memory was off. So, it is indeed possible. I am off to try to contact a few people who might have the skills to do this.
     

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