Inside the trauma/old eggs: candling and open shots, blood and embryo pics warning for squeamish!

Susan Skylark

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Apr 9, 2024
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Day 5 on my trauma/2 week old eggs. Candled again and then opened a couple from each group. Kind of interesting, nothing scientifically ground breaking but curious. The control group (0) are just eggs, no trauma, no age. The trauma eggs are simulated shipping eggs with either two (2) or three (3) days of traumatic movement. The old eggs are eggs that were stored for 2 weeks pre-incubation. I tried to use similar shelled eggs for the pictures but they didn't always cooperate. I note that several of the eggs had veins growing on the membrane or stuck to it when opened or looked clumpy on candling, I have noticed this phenomenon in most of my 5-8 day old eggs when candled (contents sluggish to move) but it doesn't seem to affect chick health or development or be a result of shipping/no shipping/or anything else, I think it is just normal as far as I can tell. Curiously the old egg is the only one without the issue, significant? I wish I had a cool computer program that could scan the extent and health of the vessels but I don't so you'll just have to guestimate with eyeballs like me. And no, my candled quail eggs DO NOT look like this to the human eye, this is what the camera sees, awesome pictures but NOT realistic, please do not use them as a guide to candling eggs!

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There you have it, whatever 'it' is! In general, incubating fresh, unshipped eggs is your best bet for healthy embryos and chicks, but that being said, even traumatized or geriatric eggs can develop into viable, if less prolifically veined, embryos. I really want to take some of these to hatch to see if chick vigor or health is affected, but I really don't have space to mess with them as the temps plunge. We'll go to Day 10 on the rest and see what happens, also have 5-6 eggs from the geriatric bunch going into the incubator every 2 days so the oldest will be 3 weeks old pre-incubation, we'll see how increasing egg age affects viability and development and early embryonic death.
 
Interesting.

I buy a lot of shipped eggs. This year has been horrible hatch rates of around 30%. I've heard that's good from how some folks have done. Years past I was always getting around 70% hatch rate.
 
So I’ve opened all my geriatric and traumatized eggs and looked at the numbers (not statistically significant!!!). The trauma eggs were a wash, a little less initial veining compared to control but 15/15 normal day 9 embryos no matter treatment group.

The geriatric eggs (2-3 weeks of age at incubation) were a bit more interesting, had development in 24 eggs, one infertile. Only 50 percent of embryos were normal or alive by day 4-9 and anything over 16 days of age were developmentally a day behind (incubator temp not an issue, trauma eggs developed fine in same little incubator at same time). Lots of day 1-2 quitters, one deformed chick. A usual hatch for me is 10 percent infertile, ten percent embryonic death, and 80 percent normal hatch. This batch was 50 percent dead by day 3! All but one egg started to develop but only half made it past day 3 and those were developmentally a day behind and one was a mutant. Overall it looks like fertility isn’t the issue but rather the egg’s ability to develop and support a healthy embryo and vasculature, development starts but in half the eggs it stops and in the other half it is slow. It would be interesting to take them to hatch but I’m not up for it at the moment.
 
Day 5 on my trauma/2 week old eggs. Candled again and then opened a couple from each group. Kind of interesting, nothing scientifically ground breaking but curious. The control group (0) are just eggs, no trauma, no age. The trauma eggs are simulated shipping eggs with either two (2) or three (3) days of traumatic movement. The old eggs are eggs that were stored for 2 weeks pre-incubation. I tried to use similar shelled eggs for the pictures but they didn't always cooperate. I note that several of the eggs had veins growing on the membrane or stuck to it when opened or looked clumpy on candling, I have noticed this phenomenon in most of my 5-8 day old eggs when candled (contents sluggish to move) but it doesn't seem to affect chick health or development or be a result of shipping/no shipping/or anything else, I think it is just normal as far as I can tell. Curiously the old egg is the only one without the issue, significant? I wish I had a cool computer program that could scan the extent and health of the vessels but I don't so you'll just have to guestimate with eyeballs like me. And no, my candled quail eggs DO NOT look like this to the human eye, this is what the camera sees, awesome pictures but NOT realistic, please do not use them as a guide to candling eggs!

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There you have it, whatever 'it' is! In general, incubating fresh, unshipped eggs is your best bet for healthy embryos and chicks, but that being said, even traumatized or geriatric eggs can develop into viable, if less prolifically veined, embryos. I really want to take some of these to hatch to see if chick vigor or health is affected, but I really don't have space to mess with them as the temps plunge. We'll go to Day 10 on the rest and see what happens, also have 5-6 eggs from the geriatric bunch going into the incubator every 2 days so the oldest will be 3 weeks old pre-incubation, we'll see how increasing egg age affects viability and development and early embryonic death.
This might be one of the most amazing things I’ve ever seen on here. Not the candling or even the embryo photos, but the actual experiment itself. I have done a lot of investigating of my failed eggs/ lost embryos that my hens hatch, but never would’ve thought to do this. Fair play!
 
I just discovered this thread; thanks for writing it @Susan Skylark . Your results on age seem consistent with published work in the area. If I remember that aright, relatively old eggs may do better if they were laid by older hens. I haven't seen any papers simulating the trauma of shipping, but there are a few that consider temperature fluctuations (which must also happen with shipped eggs).

If you don't already know them, these are good papers on the topic:
Brake et.al. Egg Handling and Storage, 1997, Poultry Science vol 76, and
Fasenko, Egg Storage and the Embryo, 2007, Poultry Science 86;
That journal is open access (currently).
 

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