Sorry for your loss. It looks like a massacre. I wonder about rats or cannibalism of the older birds. What type of floor do you have? How much room inside the coop? What is the age of the chickens? Are the cockerels trying to mate. Instead of separating pullets, if the cockerels are the culprits, they need to be separated. When the chickens go to roost, I make sure they are all on the roost, and first thing in the morning let them at at daylight. They are ready to go outside at daylight, and if they are crowded or cockerels are starting to mate, they will drive the pullets crazy. How many of each do you have? I would spend time with them in the next few days, and find out if the pullet was killed and pecked to death by the others, or if something dug in or somehow got in to your coop. Are you feeding a balanced grower feed with enough protein? I hope that you can determine what was happening, and prevent any further loss.