I believe letting them rest fat end up, in an egg carton, at room temperature (approx. 70-75 degrees) for about 24 hours is the norm. I have typically let mine set for more like 12-14 (i.e. overnight), but that is because I am a) impatient as all get out and b) tend to like to set them in the morning to have time monitor my incubator conditions before I feel confident everything is stable enough to leave them. I've been working with one horrid LG still air, though, so the monitoring doesn't really even matter...the thing does what it wants!