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3.5. Toxicological studies
The PN-M extract was subjected to toxicological studies to assess the plant's toxic effects, including different
hematological, biochemical, and physical parameters. The effect of oral administration of methanol extract of
P. nodiflora on the
haematological parameters of chicks is shown in
Table 6, while the effect of oral administration of methanol extract of
P. nodiflora on the biochemical parameters of chicks is shown in
Table 7; likewise, the effect of oral administration of methanol extract of
P. nodiflora on body organs of chicks is shown in
Table 8. Broiler chicks were selected and distributed in four experimental groups. They were orally fed with the extract for 21 days
. The chicks were physically evaluated, and the number of chicks remained the same with no abnormality. After comparing the weights of different groups, no significant variation was found. On day 21, absolute and relative weights of other body organs, including kidney, heart, spleen, trachea, liver, intestine, thymus, and lungs, were also done with no significant variation. Hematological and biochemical parameters were evaluated after collecting blood samples on days 7, 14, and 21. On account of statistical analysis,
P. nodiflora was non-toxic, as there was no significant difference in experimental values to that of the control group. Overall, we have used the methanolic extract (at different concentrations of 150 mg/kg, 200 mg/kg, 250 mg/kg, and 300 mg/kg) of
P. nodiflora plant in order to check the in-vivo toxicity. The results have indicated that the tested extract was found safer even at the higher doses of 300 mg/kg.
