Mites? Lice? How do I treat!

I agree, if you feel mites/lice are a problem, then using Permethrin is the best route to take.

You don't mention if you have a rooster and how many hens you have nor how much space in your coop/run (sq ft).

The hen's bottom in the photo looks to be mostly plucked. Do you have photos of the others and the loss around the necks?
Plucking can be due to boredom sometimes, observe and see who is doing to picking.

I also agree this hen looks to have a droopy abdomen. Since it's not covered with feathers, it can look more pronounced, but I too would consider that she may have something going on with some fluid and/or reproductive problems are starting to show up.

The biggest concern I see at this time is the broken tissue/skin. Likely this is from pressure if there's fluid and picking/plucking.
Watch that area very closely to see that it closes up. If it doesn't or gets worse, then treat as an open wound. Right now, it doesn't look like it necessarily needs treatment, but this can go south in a hurry. Look at it daily to make sure there's no maggots, weeping, etc.

View attachment 3849985
We have 28 hens and one rooster. Coop/run is rather large. I did attach pictures.

For permethrin, what is the recommended route to treat them?
 

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Ive attached some more pics of the other birds where they are losing feathers. This morning I saw them trying to pluck some feathers as well.

I picked up new bedding and permethrin. My concern is that if it's not the right type of mite treatment then we have to swap out hte bedding again. etc.

For using permethrin, what route should we go. Obviously emptying, spraying the coop and applying to them?

Also would certain mites cause certain patterns on the feathers missing and would we be able to see the mites?
Looks like they are plucking one another. What do you feed?

Is the red building behind the run also a coop or??

I like to use Permethrin to spray housing/nesting boxes, etc.
For the birds, I prefer Permethrin dust. Put the dust into a sock, tap it all over the bird like a powder puff and work the dust down through the feathers to the skin.

Yes, you should be able to see mites, especially at night. Go out with a headlamp and inspect your birds around the vents, under the wings, etc. Wipe your roosting bars with a clean white paper towel (under and around the bar), see if you get any bugs and/or red smears on the paper towel.

Mite/Lice I.D.
https://the-chicken-chick.com/poultry-lice-and-mites-identification/
 

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